Skip to main content
Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Development of novel cellular histone-binding and chromatin-displacement assays for bromodomain drug discovery

Fig. 5

Development of in situ cell extraction to monitor the displacement of endogenous TRIM24 from chromatin in cells. a Representative IF images of HeLa TRIM24-PB cells using either anti-FLAG (red) or anti-TRIM24 (green) antibodies under in situ cell extraction (+) and non-extraction (−) conditions and in the presence or absence of IACS-6558, a TRIM24 inhibitor. b Quantification of the fraction of chromatin-bound TRIM24 upon inhibitor treatment (2 h) of the above HeLa TRIM24-PB cells. c OV90 cancer cells treated as above using the same anti-TRIM24 antibody to detect endogenous TRIM24 expression and displacement upon TRIM24 inhibitor (IACS-6558) treatment. d Representative images (HeLa TRIM24-PB) showing rapid displacement of TRIM24 (red) from chromatin in response to TRIM24 inhibitor (IACS-6558) treatment. e Quantification of the inhibitor-induced displacement of TRIM24-PB from chromatin in HeLa (left graph) and non-transfected OV90 cells looking at endogenous TRIM24 (right graph)

Back to article page