Fig. 1

Genome-wide transcriptional profiling and gene selection. a Experimental design using two different cellular systems. On the left, a differentiation-based cell system and on the right a cell system based on transient ectopic MeCP2 expression. Both systems lead to a chromatin reorganization resulting in less and larger chromocenters. Both systems were used for gene expression profiling. b Results from the transcriptional profiling of the differentiation system (left) and the ectopic MeCP2 expression system (right) are presented in volcano plots (expression fold change versus statistical significance of the change). Genes selected for further analysis are depicted in blue. Selected genes shared in both conditions are outlined in orange. The expression change of the MeCP2 gene itself (11 fold) is depicted in red. As expected, the highest expression difference in low versus high MeCP2-expressing cells was MeCP2 itself. c The physical position of all selected genes on the mouse chromosomes with their full names. Arrows indicate if genes were up-, down- or unregulated during differentiation (left) or MeCP2 ectopic expression (right). Bold gene names indicate the myogenic genes according to the gene ontology classification