Fig. 2
From: Strategies for precision modulation of gene expression by epigenome editing: an overview
Designer epigenome editing systems based on dCas9. VPR refers to the effector domain, which is a tripartite design. sgRNA 2.0 refers to a scaffolding system that allows for modular effectors to be added to the sgRNAs that have been modified to contain protein-binding sites for RNA recognizing proteins. SAM refers to a synergistic activator that contains an effector domain fused to the dCas9 protein as well as the sgRNA 2.0 design to add additional designer activators. Components are not to scale as critical features are exaggerated and some non-variable features are removed