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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Role of Ccr4-Not complex in heterochromatin formation at meiotic genes and subtelomeres in fission yeast

Fig. 3

Ccr4-Not does not regulate turnover or translation of Mmi1 RNA targets. All comparisons presented are based on microarray data. a Venn diagrams comparing genes up-regulated in ccr4 and caf1 mutants relative to wild-type cells. The numbers in parentheses indicate the expected overlap if randomly generated lists of the corresponding sizes were used. The p value of the observed overlap is displayed under the diagrams. b As in A, comparing mRNAs associated with Ccr4 and those induced in ccr4 mutants. c As in (a), comparing mRNAs that interact with Ccr4 and those up-regulated in caf1 ccr4 double mutants. d Comparison of expression levels of Ccr4-associated mRNAs between pab2 and ccr4 pab2 mutants (left), and between rrp6 and rrp6 pab2 mutants. Each mRNA is represented by a dot. The lines correspond to a twofold difference between the samples. e As in (a), comparing mRNAs up-regulated in ccr4 mutant and those displaying increased stability in ccr4. f As in (a), comparing mRNAs associated with Ccr4 and those stabilized in ccr4 mutants. g Cell extracts were prepared from the indicated strains, all of which contained TAP-tagged Mei4. Samples were probed with antibodies against TAP (top) or tubulin (bottom). The interaction between TAP-Mmi1 and Red1-myc was used as a positive control. h As in (g), using strains expressing Spo5-TAP.

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