Fig. 7

Model: Ligand-dependent regulation of B cell early activation. BCR stimulation, which eventually results in proliferation, decreases several key miRNAs compared to LPS, which leads to differentiation. BCR signaling results in greater recruitment of RNA Pol II that appeared to be paused and a decrease in H3K27me3; the latter likely achieved by displacement of PRC2. BCR activation also induces certain long non-coding RNAs (lncRNA); it is possible that binding of PRC2 to lncRNAs results in its removal from the TSS region to decrease H3K27me3. BCR-induced genes have a strong preference for promoter proximal E-boxes/MYC-binding sites. LPS-induced genes show a more rapid RNA Pol II transition from initiation to elongation mode, higher H3K27me3 around the TSS, and a preference for NF-κB motifs