Skip to main content
Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Divergence of transcriptional landscape occurs early in B cell activation

Fig. 2

Stimulus Responsive RNA Pol II occupancy. a RNA Pol II occupancy normalized mean reads per million (RPM) were mapped 2 kbp relative to average TSS of genes with increased transcription shared between the two activation states (far left), preferential to BCR (middle left) or LPS (middle right), and unchanged transcription relative to the resting state (far right). Reads from the resting state are in grey, BCR in blue, and LPS in orange. b Analysis of promoter proximal and “gene body” RNA Pol II occupancy. Direct overlays of histographs from BCR induction and LPS induction groups, far left, show regions of analysis from the promoter area to further in the gene body where RNA Pol II starts from BCR (solid blue) and LPS (solid orange) starts to merge at 2250 past the TSS. Resting Pol II occupancy is shown in dotted lines for BCR induced genes (blue) and LPS-induced genes (orange). Boxplots contain the sums of mapped normalized mean RPM for each induced transcription group relative to average TSS; resting (grey), BCR (blue), LPS (orange), number of genes indicated in parentheses. Promoter proximal occupancy (p) was defined as 0.3 kbp before and after the TSS while gene body occupancy was defined from 0.3 to 2.25 kbp past the TSS. c Traveling ratios of Pol II occupancy were determined to describe average RNA Pol II movement between the promoter and interior of the gene. Ratios of mean RPMs shown at black line in boxplot from (b) were calculated; traveling ratio (TR) = (RPM mean) body(b)/(RPM mean) promoter(p). Here, we report TR relative to the resting state (TRactivation/TRrest). TRs for each cellular state; rest, BCR, and LPS are shown in Supplemental Fig. S4B

Back to article page