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Figure 9 | Epigenetics & Chromatin

Figure 9

From: A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila

Figure 9

Reduction of HP1a also changes the amounts of H3K9me3, which generally are reciprocal to those in H3K27me3. H3K9me3 levels in (A) heterochromatic regions and (B) gene promoters (hb, nullo, bnk, Ubx, h) during early development (2–4 and 3–5 h) in wild-type and embryos from Su(var)2-5 05 /CyO parents. Key to bar colors shown in (B). Time windows on the X axis. Almost all regions, heterochromatic and euchromatic gene promoters, show a switch between the 2–4 and 3–5 h windows, going from greater than or equal to wild-type to less than wild-type in embryos from Su(var)2-5 05 /CyO parents (HeT-A, TART, and nullo are the exceptions). (C) H3K9me3 levels at heterochromatic regions (1360, HeT-A, TART, and F-element) and (D) gene promoters (hb, nullo, bnk, Ubx, h) when Su(var)2-5 05 is inherited from a different parent. Mutation from the mother listed first (*/+), mutation from father listed second (+/*). Broken line across is wild-type signal level. Asterisks show genotypes significantly different from wild-type and the parental types for Su(var)2-5 05. (E) Heterochromatic region transcript levels in larvae from opposite Su(var)2-5 05 parent relative to wild-type (set to one, broken line). The RNA analysis takes into account the copy number of the repetitive elements (normalized to a single copy gene in both genotypes). All show an increase except 1360 when Su(var)2-5 05 is maternally inherited. All except F-element show a difference in level between the Su(var)2-5 05 parental origin. Asterisks show genotypes significantly different from wild-type and Su(var)2-5 05 containing progeny; (#) symbol shows significant differences between same genotype but when the mutation is inherited from the opposite parent. *P value <0.05, **P value <0.005, ***P value <0.0005 (same applies for #). Error bars represent ± SEM. NI, non-immune; ns, not significant; hb, hunchback; bnk, bottleneck; Ubx, Ultrabithorax; h, hairy.

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