Figure 6

HP1a and PcG proteins are in one complex. (A) HP1a co-IPs with proteins in PRC1 and PRC2. IP antibody listed at top (left-right: HP1, E(Z), SU(Z)12, HP1 and PC) of the figure. For each IP 1% of the total Input (In) was loaded. One-third of the total IP loaded. Proteins detected with antibody listed on left of each panel set. Suppressor of Fused (SU(FU)) is the negative control. (B, C) HP1a is not pivotal for the maintenance of PcG/trxG marks. ChIP data from wild-type and Su(var)2-5 ^05/04 third instar larvae for H3K27me3 (B) and H3K4me3 (C) at Ubx, hb, nullo, bnk, h, tsr, cta, and lt promoters. All NI data is significantly below lowest ChIP signal. Asterisks show significant changes relative to wild-type, *P value <0.05, **P value <0.005, ***P value <0.0005. Error bars represent ± SEM. IP, immunoprecipitation; In, input; HP1, heterochromatin protein 1; E(Z), enhancer of zeste; Su(z)12, suppressor of zeste 12; Su(Fu), Suppressor of Fused; H3K27me3, trimethylated histone H3 at lysine 27; Sxl, Sex-lethal; Ubx, Ultrabithorax; hb, hunchback; tsr, twinstar; bnk, bottleneck; h, hairy; lt, light; cta, concertina; NI, non-immune.