Figure 4
HP1a is at gene promoters and its reduction generally decreases the amounts of Ser-5P RNAPII at promoters. (A) HP1 ChIP data from 0–2, 1–3, and 2–4 h wild-type embryos at the promoters of Ubx, hb, Sxl, nullo, bnk, h, tsr, sgs8, cta, and lt. All show an increase after 0–2 h. (B) ChIP data for Ser-5P RNAPII from 1–3 h embryos from wild-type and Su(var)2-5 05 /CyO parents. Eight of the ten promoters show a significant decrease. The h promoter was unchanged, while the nullo promoter showed an increase. Asterisks show changes which are significant relative to Ore R (**P value <0.005, ***P value <0.0005). Error bars represent ± SEM. HP1, heterochromatin protein 1; RNAPII, RNA polymerase II; Sxl, Sex-lethal; Ubx, Ultrabithorax; hb, hunchback; tsr, twinstar; bnk, bottleneck; h, hairy; lt, light; cta, concertina.