Combinations of epigenetic features and their association with enhancer activity. (A) Each row of the diagram shows the features and activity results for 1 of the 273 tested DNA segments. The presence or absence of the 11 epigenetic features is represented by grey and white, respectively. The tested segments were organized into clusters by DBSCAN  using the binary representation of the epigenetic features. Each tested DNA segment is also categorized by activity in the transient transfection assay, denoted by a colored entry in the last three rows (red = active, pink = threshold, blue = inactive). Success rates (B) and activities (C) in enhancer assays on 273 DNA segments in each cluster formed by DBSCAN. (D) Power of SMAD1 and TAL1 binding to identify enhancers.