CycG misregulation alters ectopic sex comb phenotypes of an mxc mutant. (A) Expressivity of mesothoracic leg (L2) and metathoracic leg (L3) transformations into prothoracic leg (L1). The wild-type sex comb is marked by a white arrowhead. mxc
G46/Y males present ectopic sex comb teeth on distal L1, on L2 and L3 (black arrowheads). Only phenotypes affecting L2 and L3 were rated. These phenotypes are enhanced by CycG loss of function. (B) Penetrance of the ectopic sex combs phenotype on male legs. CycG loss of function enhances mxc
G46 induced ectopic sex combs on L2 and L3, whereas CycG gain of function suppresses ectopic sex combs on both legs. Fisher’s exact test, *P < 0.05 **P < 0.0001 (n ≥ 50).