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Figure 6 | Epigenetics & Chromatin

Figure 6

From: Multi-faceted quantitative proteomics analysis of histone H2B isoforms and their modifications

Figure 6

Polyadenylated histone isoforms show no changes in levels across the cell cycle. H2B mRNA relative expression in S-phase of the cell cycle compared to G1-phase. Canonical histones (H2B1C, H2B1D, H3.1) have much higher relative expression during S-phase compared to G1 than replication-independent histones (H3.3) and polyadenylated H2B1D. H2B1D results are from primers against the H2B1D coding sequence, and H2B1DPa results are from primers against the polyadenylation sequence. mRNA was synthesized using random hexamers and analyzed by q-RT-PCR. Bars marked with a ‘*’ indicate a P value <0.01, indicating that that histone variant was expressed at significantly higher levels in S-phase than G1-phase. Mean ± SD, n = 3.

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