Figure 2
From: Multi-faceted quantitative proteomics analysis of histone H2B isoforms and their modifications
Top-down MS of histone H2B isoforms. (A) Intact mass spectrum of HPLC-purified Jurkat H2B at the most abundant charge state, z = 17. H2B isoforms were identified based on MS/MS spectra and intact mass. Ac = acetylation. (B) Fragmentation spectrum of H2B isoforms H2B1K and H2B1H. Identified c and z ions are marked in blue (H2B1H) and red (H2B1K), respectively. The sequences at the top of the figure illustrate the extent of sequence coverage based on identified c and z ions. The inset shows magnified spectra of select c and z ion peak pairs for H2B1H and H2B1K, respectively.