Figure 9

The genes affected by 5-aza-CdR are comparable in multiple cell types and represent targets of Polycomb in ES cells. A depiction of the interactions of the genes changing expression in response to 1.0 μM 5-aza-CdR (large circles of any color) with genes induced to express in myeloma cells (blue) and a highly overlapping set of genes induced to express by 5-aza-CdR in pancreatic cancer cells and genes up-regulated in breast cancer (orange), as well as genes that represent targets of Polycomb-mediated repression in ES cells (yellow). Genes involved in multiple gene sets combine those colors. These results indicate that our results in HEK 293 T cells may represent genomic responses in human cells more generally and suggests a mechanism based on reversing Polycomb effects that may mediate the drug’s therapeutic benefits in MDS. 5-aza-CdR, 5-aza-2′-deoxycytidine.