H3K9 methylation-dependent induction of silencing. (A) Quantification of VEGF-A expression normalized to SDHA. The averages and SEM are based on two to three biological repeats. (B) ChIP-qPCR measurements of H4 acetylation at the promoter of VEGF-A. The averages and SEM are based on two to three biological repeats. (C) Time course of GFP of all experiments analyzed in this respect. The dark green bars show the average data of experiments which led to VEGF-A silencing (N = 7). The light green bars show the averages of the controls not leading to silencing (N = 14). The error bars indicate the standard errors of the mean. VEGF-A, vascular endothelial growth factor A; GFP, green fluorescence protein; d, days.