Figure 3
From: Targeted epigenome editing of an endogenous locus with chromatin modifiers is not stably maintained
Targeted deposition, spreading, and loss of histone H3K9 methylation over time. The black boxes indicate the location of the amplicons used for ChIP-qPCR (also compare with Figure 1B for a larger image of the VEGF-A gene promoter). The numbers above the bars indicate the distance of the qPCR amplicons from the zinc finger-binding site. (A) ChIP-qPCR measurements of H3K9me2 at the designated amplicons covering the promoter and gene body of VEGF-A. (B) ChIP-qPCR measurements of H3K9me3 at the designated amplicons covering the promoter and gene body of VEGF-A. The averages and SEM are based on two to three biological repeats. The corresponding P values can be found in Additional file 1: Table S2. VEGF-A, vascular endothelial growth factor A; bp, base pair; ZF-GLP-CD, zinc finger-protein lysine methyltransferase 1D-catalytic domain.