Figure 2From: Genome-wide assays that identify and quantify modified cytosines in human disease studies The effects of bisulphite conversion on different cytosine variants. Bisulphite conversion deaminates not only unmodified cytosine but also 5fC and 5caC [27] to uracil, which is then amplified and sequenced as a thymine. Both 5mC and 5hmC are resistant to bisulphite conversion and are sequenced as cytosines. While the output of bisulphite sequencing has been generally described to be the ratio of 5mC/(5mC + C), more correctly it should be described as (5mC + 5hmC)/(5mC + 5hmC + 5fC + 5caC + C), which only approximates 5mC/(5mC + C) when the other cytosine variants are in very low proportions.Back to article page