CHD1 regulates chromatin accessibility at transcription start site (TSS)-associated, intergenic, intragenic and active tRNA DNase hypersensitivity sites (DHS). (A-B) Knockdown of CHD1 reduces the accessibility of TSS-associated, intragenic and intergenic DHS sites in K562 cells. (A) Data showing the accessibility of selected loci representing non-CGI TSS DHS sites (C1ORF159 and HGB1) CGI TSS sites (PIN1 and H3F3B), intragenic DHS sites (HGB2 and CD19 intra) and two intergenic loci. Centromeric repeats were used as inaccessible controls. Data represent biological replicates and error bars standard deviation. (B) Mean accessibility values from three biological replicates for each loci tested were plotted as the percentage of accessibility in control siRNA treated cells. Differences compared to control were tested by one-way ANOVA, **P <0.01, ***P <0.001.