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Table 3 Ectopic CENP-A nucleosomes are stable octamers

From: CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells

 

SW480 bulk chromatin (n)

Recomb. H3 octamer (n)

Recomb. CENP-A octamer (n)

SW480 total CENP-A (n)

SW480 ectopic CENP-A (n)

Height a (nm)

2.53 ± 0.26 (401)

2.32 ± 0.32 (57)

2.37 ± 0.34 (57)

2.17 ± 0.35 (546)

2.46 ± 0.40 (135)

Diameter b (nm)

13.8 ± 1.4 (401)

12.1 ± 1.4 (20)

10.6 ± 1.4 (20)

14.1 ± 2.9 (546)

14.5 ± 3.0 (135)

  1. AFM measurements of aheight and bdiameter data of ectopic versus centromeric or recombinant CENP-A nucleosomes provided as average ± standard deviation, with number of particles measured indicated in parenthesis. On average, each type of experiment has three replicates.