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Figure 2 | Epigenetics & Chromatin

Figure 2

From: CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells

Figure 2

Ectopic CENP-A forms a structurally distinct type of nucleosome in colorectal cancer cells. Atomic force microscopy (AFM) analysis of nucleosomal heights from SW480 total input chromatin, in vitro reconstituted chromatin containing either H3 or CENP-A octameric nucleosomes, SW480 total CENP-A IP, and SW480 ectopic CENP-A IP. Gray graph indicates bulk octameric input, yellow indicates recombinant nucleosomes, and blue and red indicate tetrameric and octameric immunoprecipitation (IP) nucleosomes, respectively. Dashed red line indicates average octameric height. Insets contain mean ± standard deviation, and nucleosome count in each sample. P values are provided to the right of each dataset compared. Images show representative chromatin arrays, with arrowheads indicating single nucleosomes (scale bar 100 nm, data summarized in Table  3).

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Epigenetics & Chromatin

ISSN: 1756-8935