DNA methylation patterns of major satellites (mSat) and L1Md_Tf (L1) of separated pronuclei of pre- and post-replicative stages of mouse zygotes and germ cells. (A) DNA methylation patterns; for explanation, see Figure
1, (B) Absolute DNA methylation level and percentage of hemimethylated CpG dyads in relation to all methylated CpG dyads. Paternal and maternal pronuclei were separated by micromanipulation before and after DNA replication, and L1 and mSat were analyzed with deep hairpin bisulfite sequencing (DHBS). As comparison, we added DHBS data from germ cells. Only after replication was a decrease in DNA methylation found on paternal chromosomes; accompanied by an increase in hemimethylated CpG dyads on both maternal and paternal chromosomes.