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Figure 2 | Epigenetics & Chromatin

Figure 2

From: Analysis of DNA methylation acquisition at the imprinted Dlk1 locus reveals asymmetry at CpG dyads

Figure 2

Paternal allele-specific DNA methylation is acquired during post-implantation development. (A) Bisulfite mutagenesis and sequencing of DNA from B6 x CAST F1 hybrid spermatozoa. (B-F) Bisulfite mutagenesis and sequencing of DNA from B6 x CAST12 F1 hybrid 3.5 to 9.5 d.p.c. embryos. A hairpin linker approach was used to analyze the DNA methylation status of 32 potentially methylated CpG dinucleotides in sperm and 7.5 to 9.5 d.p.c. embryos. Individual circles represent one of the 32 potentially methylated CpG dinucleotides, and each paired row of circles represents the complimentary strands of an individual subclone; semi-circles to the left connect the complimentary strands. A traditional bisulfite mutagenesis approach was utilized to analyze 36 CpG sites located on the bottom, antisense strand in 3.5 d.p.c blastocysts and 6.5 d.p.c. embryos; the analyzed region includes all 29 sites contained within the Dlk1 CpG island and all 16 sites analyzed using the hairpin linker (black bar). The gap in the paternal strands represents the polymorphic site, which is not a CpG dinucleotide in Mus musculus castaneus-derived DNA. Filled circles represent methylated cytosines, open circles represent unmethylated cytosines, absent circles represent ambiguous data. Labels to the right identify the PCR subclone analyzed; letters represent independent amplification reactions, while numbers represent individual subclones. The level of methylation (y axis) on the paternal (blue) and maternal (red) strands was quantified by dividing the number of methylated residues at each CpG site by the total number of sites analyzed at that position.

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