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Table 1 Comparison between different methodologies for analyzing chromatin states with combinatorial histone PTMs

From: ChIP-less analysis of chromatin states

 

Analysis of more than two PTM states

Analysis of direct intranucleosomal connection

Ability to enrich combinatorial PTMs

Ability to quantify combinatorial PTMs

Ability to map combinatorial PTMs to the genome

Antibody -free

MARCC-quantitative mass spectrometry (this report)

✔

✔

✔

✔

✔

✔

Overlaying ChIP-sequencing tracks [14–18]

✔

   

✔

 

ChIP-re-ChIP [19, 20]

 

✔

✔

 

✔

 

ChIP-quantitative mass spectrometry [2, 21, 22]

✔

✔

 

✔

  

Middle-down histone mass spectrometry [4]

✔

✔ (within one histone tail)

 

✔

 

✔