Figure 5

Quantitative profiling of chromatin PTM patterns identifies heterochromatic signature captured by MARCC with ATRX-ADD. (A,B) Relative PTM enrichment with ATRX-ADD MARCC and AIRE-PHD MARCC. Relative enrichment was represented by log₂ of the ratio of MARCC over input. PTM states above the x-axis were enriched by MARCC, whereas PTM states in the opposite direction were depleted by MARCC. Error bars were calculated from two separate MARCC-qMS experiments. For the complete qMS data set, refer to Additional file 11: Table S1. (C) Relative enrichment of heterochromatic DNA (SAT-2) by qPCR obtained from MARCC with ATRX-ADD. ChIP with H3K9me3 antibody (ab8898) or IgG and HaloTag were included as controls.