Figure 1

Schematic representation of the Peg13 / PEG13 imprinted domain on distal mouse chromosome 15 and human chromosome 8q24. (A) Map of the Trappc9/Peg13 locus located on mouse chromosome 15, showing the location of the various imprinted transcripts (red transcripts are maternally expressed, blue are paternally expressed and black are expressed from both parental alleles; arrows represent direction of transcription) and CpG islands (green bars). (B) Schematic of the human TRAPPC9/PEG13 region on chromosome 8. (C) RNA-seq profiles throughout the human domain in brain and lymphoid tissue. The RNA peaks corresponding to PEG13 and KCNK9 are indicated in the brain track. The RNA density plots represent relative read density (normalised counts per million mapped reads as defined by the University of California Santa Cruz (USCS) sequence browser). (D) The allelic expression for the genes in the human cluster. The sequence traces for heterozygous DNA samples from brain, leucocytes and term placentae are shown, as well as the resulting RT-PCR.