Figure 4
From: HP1β-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs
Protein interactions at DNA lesions. (A) FRET results for reference interacting partners p53 (green) and 53BP1 (red). (B) FRET analysis of UBF1/2 dimerization. FRET analysis of (C) UBF1/2 (red) and γH2AX (green), (D) GFP-UBF1 (green) and 53BP1 (red), and (E) HP1β (green) and UBF1/2 (red). Studies were performed in control and γ-irradiated cells using a Leica TSC SP5 confocal microscope. For each event, 10 nuclei were analyzed in three independent experiments. IF, immunofluorescence; non-irrad., non-irradiated cells.