Figure 4

H3.3 dissociation at regulatory elements of mouse embryonic stem cells. (A) Heatmap illustrating the dissociation rates and equilibrium levels of H3.3 around chromatin modifications and protein binding sites. Average H3.3 dissociation rates and equilibrium levels within 10 kbp regions around sites of histone modifications and binding factors are represented with a color gradient (Red: high; Blue: low). (B) High H3.3 dissociation rates mark distal regulatory regions. Comparison of H3.3 dissociation rates between gene proximal and distal regulatory sites. Heatmaps showing dissociation rates and equilibrium levels of HA-H3.3 at genic and inter-genic regulatory sites. Protein binding sites located within 5 kbp of known transcripts and coding regions were considered proximal (genic) and those outside were considered distal (inter-genic). (C) Comparison of H3.3 dissociation rates at chromatin modification peaks between genic and inter-genic sites. Red and black lines denote inter-genic and genic values respectively