High H3.3 dissociation rates mark RNA Pol II binding sites. (A) H3.3 dissociation rates and equilibrium levels were measured at non-genic Pol II binding sites. Green, red, and blue colored ovals indicate Pol II binding, dissociating, and incorporating H3.3 nucleosomes at the sites. Pol II, CHD7, p300, HDAC1, HDAC2, LSD1, Mi2b, REST, and CoREST enrichment (indicated on the right of the figure) was mapped from published ChIP-Seq data (see Materials and Methods). The enrichment levels of RNA Pol II and individual chromatin remodelers were standardized to yield a distribution with mean = 0 and variance = 1 using ChIP-Seq read numbers. All plots are calculated by averaging values of the 100 bp sliding windows of the corresponding loci. (B) H3.3 dissociation rates and equilibrium levels at genic Pol II peaks. RNA-Seq libraries were used to group genes according to their transcription levels based on their FPKM values as indicated on the right. Blue arrows indicate the direction of transcription.