H3.3 dissociation rates are positively correlated with gene activity. RNA-Seq data were used to group genes according to transcription levels based on their FPKM values into Top 10% (>30 FPKM) and Top 20% (10 to 30 FPKM), medium (3 to 10 FPKM) and low (1 to 3 FPKM) and silent (<1 FPKM) genes. H3.3 dissociation rates and equilibrium levels were measured in a 1 kb window around transcription start sites, exon start sites, exon end sites and transcription termination sites. Dissociation rates were calculated based on changes in HA-H3.3 enrichment from time point 0 h to time point 6 h after DOX addition. Gray shading marks coding regions.