Figure 5

X chromosome compaction is not disrupted in condensin I or II depleted animals. (A) Quantification of the percentage of nuclear volume occupied by X in vector RNAi (n = 40), smc-4(RNAi) (n = 28), capg-2(RNAi) (n = 40), hcp-6(RNAi) (n = 40), and dpy-27(RNAi) (n = 34). Error bars indicate standard deviation. Asterisks indicate level of statistical significance by t-test analysis (three asterisks, P <0.001). (B) Quantification of the percentage of nuclear volume occupied by chromosome I in vector RNAi (n = 40), smc-4(RNAi) (n = 29), capg-2(RNAi) (n = 40), hcp-6(RNAi) (n = 40), and dpy-27(RNAi) (n = 32). Error bars indicate standard deviation. (C) FISH probe pairs across the X chromosome. The position of YAC probes (red and white boxes) used in FISH is indicated. 2D projections of 3D stacked images. Representative stained diploid nuclei of vector RNAi, smc-4(RNAi) and hcp-6(RNAi) worms. Nuclei stained with probes pairs across the X chromosome (red and white) and counterstained with DAPI (blue) to label DNA. Scale bars equal 1 μm. Boxplots indicating the distribution of 3D loci distances for vector RNAi (n = 20) and smc-4(RNAi) (n = 20) and hcp-6(RNAi) (n = 20) diploid nuclei. Boxes show the median and interquartile range of the data.