Figure 2

DCC depletion and mutations disrupt X chromosome compaction. (A, B) Adult RNAi treated hermaphrodite intestinal nuclei stained with X-paint FISH (red) to label X chromosome territories and DAPI (blue) to label DNA. (A) Representative stained nuclei after vector RNAi treatment, dpy-30(RNAi), dpy-21(RNAi), and dpy-27(RNAi). Scale bars equal 5 μm. (B) Quantification of the percentage of nuclear volume occupied by X in vector RNAi (n = 60), dpy-30(RNAi) (n = 60), dpy-21(RNAi) (n = 60), and dpy-27(RNAi) (n = 60). Error bars indicate standard deviation. Asterisks indicate level of statistical significance by t-test analysis (three asterisks, P <0.001). (C) Quantification of the percentage of nuclear volume occupied by X in wild type (n = 36), dpy-30(y228) (n = 24), and dpy-21(e428) (n = 21). Error bars indicate standard deviation. Asterisks indicate level of statistical significance by t-test analysis (three asterisks, P <0.001). (D, E) Depletion or mutations of DCC leads to no difference in chromosome I size. (D) Quantification of the percentage of nuclear volume occupied by chromosome I in vector RNAi (n = 40), dpy-30(RNAi) (n = 40), dpy-21(RNAi) (n = 40), and dpy-27(RNAi) (n = 40). Error bars indicate standard deviation. (E) Quantification of the percentage of nuclear volume occupied by chromosome I in wild type (n = 25), dpy-30(y228) (n = 25), and dpy-21(e428) (n = 25). Error bars indicate standard deviation.