Figure 1

The absence of the DCC leads to enlarged X territories. (A) Chromosome-paint 3D FISH measures the volumes of chromosome X and I territories. Representation of how the percentage of nuclear volume occupied by the X or chromosome I paint were quantified in a single nucleus. (B) C. elegans has five pairs of autosomes and one pair of sex chromosomes. Hermaphrodites have 12 chromosomes with a total genome sequence of 194 Mb. The X chromosomes occupy 18% and chromosome I occupies 14% of the total hermaphrodite genome size. Males have 11 chromosomes with a total genome sequence of 177 Mb. The single X chromosome occupies 10% and chromosome I occupies 16% of the total male genome size. (C-D) Adult hermaphrodite and male intestinal nuclei stained with X-paint FISH (red) to label X chromosome territories and DAPI (blue) to label DNA. (C) Representative stained nuclei of wild type hermaphrodites, male him-8(e1489), hermaphrodite and male him-8(e1489) dpy-27 RNAi treated animals. Scale bars equal 5 μm. (D) Quantification of the percentage of nuclear volume occupied by X in wild type hermaphrodites (n = 40), male him-8(e1489) (n = 40), hermaphrodite dpy-27(RNAi) (n = 60), and male him-8(e1489) dpy-27(RNAi) (n = 22). Error bars indicate standard deviation. Asterisks indicate level of statistical significance by t-test analysis (one asterisk, P <0.05; two asterisks, P <0.01; three asterisks, P <0.001). (E, F) Adult hermaphrodite and male intestinal nuclei stained with chromosome I paint FISH (red) to label chromosome I territories and DAPI (blue) to label DNA. (E) Representative stained nuclei of wild type hermaphrodites, male him-8(e1489) and hermaphrodite dpy-27 RNAi treated animals. Scale bars equal 5 μm. (F) Quantification of the percentage of nuclear volume occupied by chromosome I in wild type hermaphrodites (n = 40), male him-8(e1489) (n = 40) and hermaphrodite dpy-27(RNAi) (n = 40). Error bars indicate standard deviation.