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Figure 5 | Epigenetics & Chromatin

Figure 5

From: Role of distinct surfaces of the G9a ankyrin repeat domain in histone and DNA methylation during embryonic stem cell self-renewal and differentiation

Figure 5

G9a, but not the histone H3 binding surface of the ankyrin repeat (ANK) domain, is required for efficient repression of SSEA-1 expression during embryonic stem cell (ESC) differentiation induced by retinoic acid (RA). Fluorescence-activated cell sorting (FACS) analysis of cell surface protein SSEA-1 expression in the indicated mESC lines that were untreated (0) or treated with RA for 3 or 7 days. Shaded histograms show nonspecific staining (obtained when staining exclusively with secondary antibody) and open histograms show specific SSEA-1 staining (staining with both primary and secondary antibodies).

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Epigenetics & Chromatin

ISSN: 1756-8935