Figure 2
TGCCGC motif analyses. (A) DREME results for the DMR CpG islands (CGIs) (n = 28) versus oocyte-methylated CGIs (n = 1,013) comparison. Motif: a regular expression representation of the motif in IUPAC format. Logo: continuous motif representation (y-axis is information content in bits) on the forward strand. RC: reverse complement. E-value: statistical significance measure. (B) E-value distributions of DREME-identified motifs in DMR CGIs versus oocyte-methylated and oocyte-unmethylated CGIs. X-axis: E-value bins on a log10 scale, with text labels referring to the bin centres. Y-axis: number of motifs with an E-value in the respective bin. (C) Presence statistic for the TGCCGC motif per CGI category with and without +/- 1 kbp flanking sequences (x-axis). The y-axis represents the percentage of CGIs containing at least one occurrence of the motif. The numbers underneath each category are the total number of sequences in each CGI group. (D) TGCCGC density per CGI category with and without +/- 1 kbp flanking sequences. The primary y-axis represents density as occurrences per 1 kbp, while the secondary y-axis shows density relative to the background density in the whole mouse genome (NCBI build 37). DMRs, maternal permanent gDMR CGIs.