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Figure 3 | Epigenetics & Chromatin

Figure 3

From: G9a co-suppresses LINE1 elements in spermatogonia

Figure 3

G9a co-suppresses L1 in spermatogonia. (A) Immunofluorescence using anti-L1 ORF1 antibody on adult testis sections of the indicated genotypes are shown. The inset highlights a crop section containing spermatogonia. (B) Immunofluorescence using anti-L1 Orf1 and anti-PLZF antibodies on spermatogonia from testis sections of the indicated genotypes are shown. (C) Quantification of L1 Orf1 signal from immunofluorescence of spermatogonia cells of the indicated genotype is shown. Bars represent mean ± s.e.m. (n = 20 to 40 cells). (D) Northern blot containing testicular RNA of the indicate genotypes probed with an L1 probe is shown. Full-length L1 transcripts are indicated. 28S RNA is shown as a loading control. The two G9aCKO;MiliKO samples represent biological replicates. (E) qRT-PCR measurement of L1 transcripts from testicular RNA of the indicated genotypes is shown. Bars represent mean ± s.e.m. (n = 3). (F-G) Immunofluorescence using anti-L1 ORF1 (F), anti-IAP (G) and anti-γH2AX antibodies on spermatogonia from testis sections of the indicated genotypes are shown. (H) Quantification of IAP Gag protein signal from immunofluorescence of spermatogonia cells of the indicated genotype is shown. Bars represent mean ± s.e.m. (n = 20 to 40 cells). (I) Northern blot containing testicular RNA of the indicate genotypes probed with an IAP probe is shown. Full-length IAP transcripts are indicated. 28S RNA is shown as a loading control. The two G9aCKO; MiliKO samples represent biological replicates. (J) qRT-PCR measurement of IAP transcripts from testicular RNA of the indicated genotypes is shown. Bars represent mean ± s.e.m. (n = 3).

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