Expression and conditional ablation of G9a in the adult testis. (A) Immunofluorescences using anti-G9a antibody on wild type germ cells from adult testis sections are shown. Dashed lines outline the indicated cell type. (B) Overview of deletion protocol for inducible G9a ablation and analysis. (C-F) Immunofluorescences using anti-G9a (C), anti-H3K9me2 (D), anti-GLP (E) and anti-H3K9me3 (F) antibodies on spermatogonia from testis sections of the indicated genotypes are shown. (G) Scheme indicating the repressive L1 mechanisms functioning in spermatogonia of the respective genotypes.