Figure 3

H3K9me2 nucleation and effect on chromatin accessibility is biased towards the low deamination, mostly unmethylated CGIs. (A) H3K9me2 nucleation sites (blue) are biased towards CGIs with low rates of C- > T deamination (green) as compared to CGIs that evolutionarily show G/C gain (red). (B) The increase in chromatin accessibility is more dramatic at the CGIs with low rates of C- > T deamination (green) as compared to CGIs that evolutionarily have G/C gain (red). (C) DNA methylation levels in CD34⁺ HSPCs for CGIs with low rates of C- > T deamination (green) and CGIs displaying G/C gain (red) indicate that the CGIs with low rates of C- > deamination are mostly unmethylated while the GCIs displaying evolutionary gain of G/C content are mostly methylated.