H3K9me2 is associated with condensed chromatin and removal of H3K9me2 through UNC0638 reverses chromatin condensation. (A) A flow diagram summarizing the study. See text for details. (B) Genome browser screen shot of the HOX locus on chromosome 7 indicating a mutual exclusivity of H3K9me2 and chromatin accessibility as measured with FAIRE as well as an increase in chromatin accessibility upon loss of H3K9me2 following UNC0638 treatment. (C) Closer look at the HOXA9 and HOXA10 reveals the relationship between H3K9me2 and chromatin accessibility as measured by FAIRE. (D) Scatter plot of normalized FAIRE signals at all H3K9me2 sites in HSPCs for UNC0638- and DMSO-treated samples reveals a general increase of chromatin accessibility at H3K9me2 sites upon UNC0638 treatment. (E) Fold change (UNC0638/DMSO) for FAIRE signals at H3K9me2 sites as compared to randomly selected regions. Sites of H3K9me2 nucleation in HSPCs are unique in their response to UNC0638.