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Figure 7 | Epigenetics & Chromatin

Figure 7

From: Phosphorylation and arginine methylation mark histone H2A prior to deposition during Xenopus laevis development

Figure 7

Nucleosome immunoprecipitation demonstration of S1ph and R3me1/2 s coenrichment with active and repressive histone PTMs. (A) Chromatin from A549 cells was digested with micrococcal nuclease and immunoprecipitated with anti-H3K4me3, H3K9me3, and H3K27me3 antibodies. Precipitated histones were blotted for H2A, H3, H4, H3K4me3, K9me3, K27me3, S1ph, R3me1, and R3me2s as indicated. (B) Chromatin from stage 13 Xenopus embryos was digested with micrococcal nuclease and immunoprecipitated with control IgG, anti-H3K4me3, and H3K9me3 antibodies. Precipitated histones were blotted for H2A, H3, H4, H3K4me3, K9me3, S1ph, R3me1, and R3me2s as indicated.

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Epigenetics & Chromatin

ISSN: 1756-8935