Figure 1
From: DNA methylation dynamics during ex vivo differentiation and maturation of human dendritic cells
DNA methylation changes occur at non-CGI and transcription factor binding sites during dendritic cell (DC) differentiation and maturation. A) Heatmap showing the CG sites with greater than 10% methylation difference between monocytes and immature dendritic cells (iDCs) among all samples. B) Heatmap showing the CG sites with greater than 10% methylation difference between iDCs and mature dendritic cells (mDCs) among all samples. C) Global DNA methylation levels measured by 5mC ELISA in monocytes, sorted iDCs and sorted mDCs used for microarray analysis. Four technical replicates were used in each cell type, and results are shown as mean ± SD. *P <0.05, **P <0.01, n.s., nonsignificant, paired t test. D) Examples of potential transcription factor binding sites within 50 bp around demethylated CG sites.