Immunofluorescence showing localisation of 5caC between e18.5 and e21.5. One seminiferous cord is shown outlined in each image; germ cells are indicated by white arrows and somatic cells within the seminiferous cord are indicated by blue arrows. Images show that 5caC is undetectable in germ cells between e18.5-e21.5 (A to D). Propidium iodide was used as a nuclear counterstain (red). 5caC was detectable in somatic cells throughout the time course. Scale bar = 50 μm.