Figure 6
Immunofluorescence showing localisation of 5fC between e18.5 and e21.5. One seminiferous cord is shown outlined in each image; germ cells are indicated by white arrows and somatic cells within the seminiferous cord are indicated by blue arrows. Images show that 5fC is undetectable in germ cells between e18.5–e21.5 (A to D). Propidium iodide was used as a nuclear counterstain (red). 5fC was detectable in somatic cells throughout the time course. Scale bar = 50 μm.