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Table 6 List and sequences of primers employed for qPCR assay

From: PAT-ChIP coupled with laser microdissection allows the study of chromatin in selected cell populations from paraffin-embedded patient samples

Organism Gene Forward primer sequence Reverse primer sequence bp from TSS
     Start End
Mouse Actb 5′-TTCCAGGCCCTCCCTCAT-3′ 5′-GAACTTCCTGTCACAGTAGCAGGA-3′ −2,991 −2,891
Mouse Actb 5′-GACCTCTATGCCAACACAGTGC-3′ 5′-ATGGTGCTAGGAGCCAGAGC-3′ +2,548 +2,648
Mouse Gapdh 5′-CAGATCAGCTGCCTGTGTGG-3′ 5′-GAAAGTCAGCCGAGCTGCATA-3′ −2,986 −2,886
Mouse Gapdh 5′-TCTTTCCCTTAAACAGGCCCA-3′ 5′-CGTGGTTCACACCCATCACA-3′ +2,528 +2,628
Human Vcl 5′-ATGCCAGTGTTTCATACGCG-3′ 5′-CGCCCTCCTCGTGCATTAT-3′ +94 +184
Human Gapdh 5′-TTCGCTCTCTGCTCCTCCTG-3′ 5′-CCTAGCCTCCCGGGTTTCTC-3′ +95 +185
Human Hapln1 5′-TCGGATGCTCTCAAGTTCTGC-3′ 5′-TCGCCCAGAGACAAACTTAAGG-3′ +177 +267
Human Tnsrsf11b 5′-GTGAAGGGAACAGTGCTCCG-3′ 5′-GCCCGTGCTATTCTGCATTC-3′ −540 −420
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Epigenetics & Chromatin

ISSN: 1756-8935