Table 6 List and sequences of primers employed for qPCR assay
Organism | Gene | Forward primer sequence | Reverse primer sequence | bp from TSS | |
|---|---|---|---|---|---|
Start | End | ||||
Mouse | Actb | 5′-TTCCAGGCCCTCCCTCAT-3′ | 5′-GAACTTCCTGTCACAGTAGCAGGA-3′ | −2,991 | −2,891 |
Mouse | Actb | 5′-GACCTCTATGCCAACACAGTGC-3′ | 5′-ATGGTGCTAGGAGCCAGAGC-3′ | +2,548 | +2,648 |
Mouse | Gapdh | 5′-CAGATCAGCTGCCTGTGTGG-3′ | 5′-GAAAGTCAGCCGAGCTGCATA-3′ | −2,986 | −2,886 |
Mouse | Gapdh | 5′-TCTTTCCCTTAAACAGGCCCA-3′ | 5′-CGTGGTTCACACCCATCACA-3′ | +2,528 | +2,628 |
Human | Vcl | 5′-ATGCCAGTGTTTCATACGCG-3′ | 5′-CGCCCTCCTCGTGCATTAT-3′ | +94 | +184 |
Human | Gapdh | 5′-TTCGCTCTCTGCTCCTCCTG-3′ | 5′-CCTAGCCTCCCGGGTTTCTC-3′ | +95 | +185 |
Human | Hapln1 | 5′-TCGGATGCTCTCAAGTTCTGC-3′ | 5′-TCGCCCAGAGACAAACTTAAGG-3′ | +177 | +267 |
Human | Tnsrsf11b | 5′-GTGAAGGGAACAGTGCTCCG-3′ | 5′-GCCCGTGCTATTCTGCATTC-3′ | −540 | −420 |