PAT-ChIP coupled with laser microdissection allows the study of chromatin in selected cell populations from paraffin-embedded patient samples

Table 6 List and sequences of primers employed for qPCR assay

Organism	Gene	Forward primer sequence	Reverse primer sequence	bp from TSS
				Start	End
Mouse	Actb	5′-TTCCAGGCCCTCCCTCAT-3′	5′-GAACTTCCTGTCACAGTAGCAGGA-3′	−2,991	−2,891
Mouse	Actb	5′-GACCTCTATGCCAACACAGTGC-3′	5′-ATGGTGCTAGGAGCCAGAGC-3′	+2,548	+2,648
Mouse	Gapdh	5′-CAGATCAGCTGCCTGTGTGG-3′	5′-GAAAGTCAGCCGAGCTGCATA-3′	−2,986	−2,886
Mouse	Gapdh	5′-TCTTTCCCTTAAACAGGCCCA-3′	5′-CGTGGTTCACACCCATCACA-3′	+2,528	+2,628
Human	Vcl	5′-ATGCCAGTGTTTCATACGCG-3′	5′-CGCCCTCCTCGTGCATTAT-3′	+94	+184
Human	Gapdh	5′-TTCGCTCTCTGCTCCTCCTG-3′	5′-CCTAGCCTCCCGGGTTTCTC-3′	+95	+185
Human	Hapln1	5′-TCGGATGCTCTCAAGTTCTGC-3′	5′-TCGCCCAGAGACAAACTTAAGG-3′	+177	+267
Human	Tnsrsf11b	5′-GTGAAGGGAACAGTGCTCCG-3′	5′-GCCCGTGCTATTCTGCATTC-3′	−540	−420