Table 1 List of antibodies for cell type identification in retina and brain and for recognition of retinal structures
From: DNA methylation reader MECP2: cell type- and differentiation stage-specific protein distribution
Antibody abbreviation | Antigen transmitter/protein | Recognized cells/structures | Source, catalogue number |
|---|---|---|---|
ChAT | Choline acetyl transferase | Cholinergic amacrine cells | Millipore, AB144P |
Calbindin | Calcium-binding protein 28 kD | Horizontal cells | SWANT, #300 |
GFAP | Glial fibrillary acidic protein | Astroglia | Sigma, G 3893 |
GABA | Gamma aminobutyric acid | Amacrine, horizontal cells | Sigma, A 2052 |
GABA-A α1 | GABA receptor subunit α1 | Bipolar, amacrine, and ganglion cell processes in IPL | Millipore, #06-868 |
GABA-C | GABA receptor subunit ρ1 | Synapses in IPL | R. Enz, MPI for Brain Research, Frankfurt |
GAT | GABA transporter | Presynaptic terminals | Abcam, ab426 |
GAD-65 | Glutamic acid decarboxylase (GABA-synthesizing enzyme) | Amacrine, horizontal cells | Chemicon, MAB351R |
GAD-67 | Glutamic acid decarboxylase (GABA-synthesizing enzyme) | Amacrine, horizontal cells | Abcam, ab26116 |
GS | Glutamine synthetase | Müller cells (astroglia) | BD Biosciences, #610517 |
GluR3 | Glutamate-gated ion channel (glutamate receptor 3) | Synapses in IPL and OPL | Santa Cruz, sc-7612 |
GlyT1 | Glycine transporter 1 | Amacrine cells | Chemicon, AB1770 |
Iba 1 | Ionized calcium binding adaptor molecule 1 | Microglia/macrophage | Wako, #019-19741 |
MAP2 | Microtubule-associated protein 2 | Neurons | Sigma, M1406 |
NR1C2 | NMDA receptor 1 splice variant C2 | IPL and OPL synapses | Chemicon, AB5050P |
PKCα | Protein kinase C | Rod bipolar cells | Sigma, P 4334 |
PKA II β | Human protein kinase A, regulatory subunit II beta | Cone bipolar cells | BD Biosciences, #54720 |
PSD-95 | Postsynaptic density protein 95 | Photoreceptors (rods and cones) synapse marker | Dianova, MA1-046 |
SV2 | Membrane of synaptic vesicles | General synapse marker | DSHB, SV2-a1 |
TH | Tyrosine hydroxylase | Dopaminergic amacrine cells | Immunostar, #22941 |
VGLUT1 | Vesicular glutamate transporter 1 | IPL and OPL synapses | Millipore, MAB5502 |
VGLUT3 | Vesicular glutamate transporter 3 | Amacrine cells | Millipore, AB5421 |
Znp-1 (Syt2) | Synaptotagmin II | Cone bipolar cells | Zebrafish International Resource Center, University of Oregon, Eugene, OR, Znp-1 |