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Table 1 List of antibodies for cell type identification in retina and brain and for recognition of retinal structures

From: DNA methylation reader MECP2: cell type- and differentiation stage-specific protein distribution

Antibody abbreviation Antigen transmitter/protein Recognized cells/structures Source, catalogue number
ChAT Choline acetyl transferase Cholinergic amacrine cells Millipore, AB144P
Calbindin Calcium-binding protein 28 kD Horizontal cells SWANT, #300
GFAP Glial fibrillary acidic protein Astroglia Sigma, G 3893
GABA Gamma aminobutyric acid Amacrine, horizontal cells Sigma, A 2052
GABA-A α1 GABA receptor subunit α1 Bipolar, amacrine, and ganglion cell processes in IPL Millipore, #06-868
GABA-C GABA receptor subunit ρ1 Synapses in IPL R. Enz, MPI for Brain Research, Frankfurt
GAT GABA transporter Presynaptic terminals Abcam, ab426
GAD-65 Glutamic acid decarboxylase (GABA-synthesizing enzyme) Amacrine, horizontal cells Chemicon, MAB351R
GAD-67 Glutamic acid decarboxylase (GABA-synthesizing enzyme) Amacrine, horizontal cells Abcam, ab26116
GS Glutamine synthetase Müller cells (astroglia) BD Biosciences, #610517
GluR3 Glutamate-gated ion channel (glutamate receptor 3) Synapses in IPL and OPL Santa Cruz, sc-7612
GlyT1 Glycine transporter 1 Amacrine cells Chemicon, AB1770
Iba 1 Ionized calcium binding adaptor molecule 1 Microglia/macrophage Wako, #019-19741
MAP2 Microtubule-associated protein 2 Neurons Sigma, M1406
NR1C2 NMDA receptor 1 splice variant C2 IPL and OPL synapses Chemicon, AB5050P
PKCα Protein kinase C Rod bipolar cells Sigma, P 4334
PKA II β Human protein kinase A, regulatory subunit II beta Cone bipolar cells BD Biosciences, #54720
PSD-95 Postsynaptic density protein 95 Photoreceptors (rods and cones) synapse marker Dianova, MA1-046
SV2 Membrane of synaptic vesicles General synapse marker DSHB, SV2-a1
TH Tyrosine hydroxylase Dopaminergic amacrine cells Immunostar, #22941
VGLUT1 Vesicular glutamate transporter 1 IPL and OPL synapses Millipore, MAB5502
VGLUT3 Vesicular glutamate transporter 3 Amacrine cells Millipore, AB5421
Znp-1 (Syt2) Synaptotagmin II Cone bipolar cells Zebrafish International Resource Center, University of Oregon, Eugene, OR, Znp-1
  1. Millipore (Billerica, MA, USA), Swant (Marly, Switzerland), Sigma-Aldrich (St. Louis, MO, USA), Abcam (Cambridge, UK), Chemicon (Billerica, USA), BD Biosciences (Franklin Lakes, NJ, USA), Santa Cruz (Dallas, TX, USA), Wako (Richmond, VA, USA), Dianova (Hamburg, Germany), DSHB (University of Iowa, IA, USA).