Analysis of expression of MBD proteins in cultured fibroblasts and tissues from Mecp2-/yand wild-type mice. (A) Relative transcription level of MBD proteins in wild-type embryonic fibroblasts (MEF W9) and adult fibroblasts established from Mecp2-/y and littermate Mecp2lox/y mice. Values are normalized to the Mecp2 transcript in the embryonic fibroblasts. Note that the mRNA levels in the embryonic and adult fibroblasts differ, whereas no difference in transcription was detected between Mecp2-/y and Mecp2lox/y genotypes. Relative transcription level of MBD proteins in the brain (B) and liver (C) from Mecp2-/y and littermate Mecp2wt mice. Values are normalized to the Mecp2 transcript in the respective Mecp2wt tissue. Note that there is no upregulation of MBD protein genes upon deletion of Mecp2. Results of real-time PCR analysis of two (for tissues) and three (for cells) biological replicates are given as mean ± S.E.M. Statistical difference between values was estimated by t test; statistically significant differences in transcription levels are marked by asterisks (*<0.05; **<0.01).