Presence of MECP2 in different cell types of adult mouse tissues. (A) List of the studied tissues and cell types; the strength of MECP2 signal is shown by the number of plus signs (1 to 3). *Tissues studied at six developmental age points (P0, P2, P5, P9, and P14). **Satellite cells were negative at P0–P14. ***Dermal fibroblasts were negative at P0–P5. ****Fibroblasts of dermal papilla were negative at P0 and weakly positive at P2; see also Figure 5D. Examples of mouse tissues after MECP2 staining: intestine (B, C), hair (D), muscles (E, F, G), and liver (H). In (C), empty arrows point at MECP2-negative gastroepithelial cells in colon crypt; empty arrowheads point at positive smooth muscle nucleus beneath the gastrodermis. In (D), solid arrows mark fibroblasts of the dermal papilla; solid arrowheads mark matrix keratinocytes of the hair bulb. For comparison of MECP2 staining in mouse and rat tissues, see Additional file4. Single confocal sections. Scale bars: (B) 50 μm, (C, D) 10 μm, (E, F, G, H) 5 μm.