Figure 3

Retinas of Mecp2^-/ymice show no apparent defects. (A) Positioning of amacrine cells, rod bipolar cells, and photoreceptor synapses is similar in retinas of Mecp2^-/y and Mecp2^lox/y littermates. Other 14 markers for retinal cell types, synapses, and neurotransmitters are shown in Additional file2. (B) Similar distribution of a histone modification typical of euchromatin (H3ac) in Mecp2^-/y and Mecp2^wt littermate retinas; nuclei with conventional (ganglion and INL cells) and inverted (rods) architecture are shown. (C) The proportions of rod nuclei with two or more chromocenters were scored in retinas of two Mecp2^-/y and one Mecp2^wt littermate at two age points, P30 and P53 (C1). At P53, nearly all nuclei have a single chromocenter. Average proportions of rods with two or less chromocenters were not significantly different between the two genotypes. Errors bars are the 95% confidence intervals. Rod nuclei with two (C2) and one (C3) chromocenter. Scale bars: (A) 25 μm, (B) 5 μm, (C) 2 μm.