Microglial cells (A) have no detectable MECP2 compared to astroglia (B) and neurons (C). (A, B) MECP2 detection in brain cortex, cerebellum, spinal cord, and retina combined with microglial (A) and astroglial (B) cell type-specific staining. Overlays of 4',6-diamidino-2-phenylindole (DAPI) staining (red) with markers for microglia (Iba-1) and astroglia (GFAP) are shown in left columns as projections of short stacks. Middle and right columns show single optical sections (zoomed in) for DAPI and MECP2. Non-marked cells in the same images are predominantly neurons and strongly express MECP2. Red outlines in the right column images trace the shape of the nuclei of interest. (C) Neurons from cerebellum – Purkinje cells (C1) and granular cells (C2) demonstrate strong MECP2 staining in chromocenters and moderate staining of the nucleoplasm in a single confocal section. Scale bars: (A,B) 10 μm, (C) 5 μm.