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Table 1 H1 regulates hematopoietic tumor formation caused by hyperactive JAK

From: Drosophila linker histone H1 coordinates STAT-dependent organization of heterochromatin and suppresses tumorigenesis caused by hyperactive JAK-STAT signaling

Genotype

N

Tumor index

p value

H1 expression

hop Tum-l /+

180

0.67

 

n.d.

hop Tum-l /+; pINT1-Nau/Act-GAL4

68

0.69

 

(100%)

pINT1-H1 6F /+; Tub-GAL4/+

100

0.00

 

Approximately 5%

hop Tum-l /+; pINT1-H1 4M /Act-GAL4

82

1.26

0.005

Approximately 30%

hop Tum-l /+; UAS-H1/Act-GAL4

214

0.35

0.003

n.d.

hop Tum-l /+; UAS-STAT/Act-GAL4

68

2.76

<0.0001

n.d.

hop Tum-l /+; UAS-STAT(Y704F)/Act-GAL4

81

0.67

 

n.d.

  1. hopTum-l/FM7C flies were mated to wild type flies; hopTum-l/FM7C; Actin-GAL4/CyO flies were mated to homozygous transgenic pINT1-Nau, pINT1-H14M, UAS-H1, UAS-STAT92E or UAS-STAT92E(Y704F) flies; homozygous transgenic pINT1-H16F flies were mated to Tubulin-GAL4/TM3, Sb flies. All crosses were set at 29°C. The tumor index in adult progeny of the appropriate genotype was calculated as described previously[18]. p values were calculated by Mann-Whitney test. N number of flies scored. Estimated H1 protein expression is presented as percentage (%) of wild type level; n.d. not determined.