Skip to main content

Table 1 H1 regulates hematopoietic tumor formation caused by hyperactive JAK

From: Drosophila linker histone H1 coordinates STAT-dependent organization of heterochromatin and suppresses tumorigenesis caused by hyperactive JAK-STAT signaling

Genotype N Tumor index p value H1 expression
hop Tum-l /+ 180 0.67   n.d.
hop Tum-l /+; pINT1-Nau/Act-GAL4 68 0.69   (100%)
pINT1-H1 6F /+; Tub-GAL4/+ 100 0.00   Approximately 5%
hop Tum-l /+; pINT1-H1 4M /Act-GAL4 82 1.26 0.005 Approximately 30%
hop Tum-l /+; UAS-H1/Act-GAL4 214 0.35 0.003 n.d.
hop Tum-l /+; UAS-STAT/Act-GAL4 68 2.76 <0.0001 n.d.
hop Tum-l /+; UAS-STAT(Y704F)/Act-GAL4 81 0.67   n.d.
  1. hopTum-l/FM7C flies were mated to wild type flies; hopTum-l/FM7C; Actin-GAL4/CyO flies were mated to homozygous transgenic pINT1-Nau, pINT1-H14M, UAS-H1, UAS-STAT92E or UAS-STAT92E(Y704F) flies; homozygous transgenic pINT1-H16F flies were mated to Tubulin-GAL4/TM3, Sb flies. All crosses were set at 29°C. The tumor index in adult progeny of the appropriate genotype was calculated as described previously[18]. p values were calculated by Mann-Whitney test. N number of flies scored. Estimated H1 protein expression is presented as percentage (%) of wild type level; n.d. not determined.