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Figure 1 | Epigenetics & Chromatin

Figure 1

From: Drosophila linker histone H1 coordinates STAT-dependent organization of heterochromatin and suppresses tumorigenesis caused by hyperactive JAK-STAT signaling

Figure 1

Hyperactive JAK affects chromocenter formation in Drosophila polytene chromosomes. Polytene chromosomes of salivary gland cells from L3 larvae were analyzed by indirect immunofluorescence (IF) staining with antibodies against HP1 (green) and H3K9Me2 (red). DNA was stained with DAPI (blue). Top, wild type polytene chromosomes have a uniform regular structure of bands and interbands with a single chromocenter characterized by overlapping intense HP1 and H3K9Me2 staining. Middle, hopTum-l polytene chromosomes have an abnormal morphology with disrupted polytene chromosome structure and dispersed HP1 foci. A single chromocenter cannot be discerned by DAPI or HP1 staining. H3K9Me2 staining overlaps with HP1-positive foci. Bottom, H1-depleted polytene chromosomes have an abnormal morphology with disrupted polytene chromosome structure and dispersed HP1 foci. A single chromocenter cannot be discerned by DAPI or HP1 staining. H3K9Me2 staining is strongly reduced. Scale bar represents 10 μm.

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